PODS® Human BDNF

CellGS Catalogue Code

Product Name

Pack Size

Price (RMB)

PPH1-50

PODSHuman BDNF

50 million

¥2,295

PPH1-250

PODSHuman BDNF

250 million

¥7,038

PPH1-1000

PODSHuman BDNF

1 billion

¥23,562

将生长因子包埋进蛋白质晶体（多角体蛋白）中，多角体蛋白能够保护包埋的生长因子，并维持其稳定性与生物活性，进而持续释放到细胞培养系统中。

PODS多角体蛋白与BDNF共结晶。BDNF(Brain-derived neurotrophic factor, 脑源性神经营养因子)是一种神经生长因子，它与低亲和力神经生长因子受体(LNGFR)和原肌球蛋白受体激酶B (TrkB)结合，支持神经元生长和存活。海马区BDNF(Brain-derived neurotrophic factor, 脑源性神经营养因子)的表达对长期记忆的储存和学习至关重要。BDNF(Brain-derived neurotrophic factor, 脑源性神经营养因子)的某些蛋白结构域与NGF和另一种神经营养因子NT-3(神经营养因子-3)的结构域相同。人、小鼠、大鼠和猪BDNF(脑源性神经营养因子)是交叉反应的。

PODS BDNF Product Details

Length

164 aa

Molecular Weight

37.6 kDa

Structure

Dimer

Source

Spodoptera frugiperda (Sf9) cell culture

Accession Number

P23560

Endotoxin Level

0.06 EU/ml as measured by gel clot LAL assay

Formulation

PODS® were lyophilized from a volatile solution

Reconstitution

PODS® co-crystals may be reconstituted at 200 million co-crystals/ml in water. 20% glucose has a buoyant density closer to PODS® co-crystals and can be useful for aliquoting.

PODS® co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8).

Stability and Storage

Upon receipt, store at 4°C. PODS® co-crystals are stable for at least 1 year when dry and 6 months when resuspended.

PODS® co-crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS® co-crystals generates the same peak dose as 3.3 μg of standard recombinant protein. However, at 5 days following the start of seeding the PODS® co-crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS® co-crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we suggest using 50 million PODS® co-crystals in place of 3.3 μg of standard growth factor as a starting point.

To control for cross-reactivity with cells or as a negative control, we recommend using PODS® growth factors alongside PODS® Empty crystals, as the latter do not contain or release cargo protein.

MADVAGTSNR DFRGREQRLF NSEQYNYNNS KNSRPSTSLY KKAGLMHSDP ARRGELSVCD SISEWVTAAD KKTAVDMSGG TVTVLEKVPV SKGQLKQYFY ETKCNPMGYT KEGCRGIDKR HWNSQCRTTQ SYVRALTMDS KKRIGWRFIR IDTSCVCTLT IKRGR

Brain-derived neurotrophic factor, neurotrophin, abrineurin, 脑源性神经营养因子

更多PODS 相关产品：PODS 生长因子

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